SPOC domain-containing protein 1 regulates the proliferation and apoptosis of human spermatogonial stem cells through adenylate kinase 4

Figure 4 Influence of SPOC domain-containing protein 1 knockdown on the proliferation of human spermatogonial stem cells. A: Quantitative PCR results show SPOC domain-containing protein 1 (SPOCD1) mRNA levels in a human spermatogonial stem cell line after transfection with SPOCD1-small interfering RNA (siRNA) 1-, 2-, and 3; B and C: Western blotting shows the changes of SPOCD1 protein after transfection with SPOCD1-siRNA; D: The Cell Counting Kit-8 assay shows the proliferation of human SSCs transfected with negative control (NC)-siRNA and SPOCD1-siRNA 2; E and F: Protein levels of promyelocytic leukemia zinc finger (PLZF), cyclin D1 (CCND1), proliferating cell nuclear antigen (PCNA), and Thy-1 cell surface antigen (THY1) after transfection with NC-siRNA and SPOCD1-siRNA 2; G and H: The percentage of 5-ethynyl-2’-deoxyuridine (EdU)-positive cells after transfection with NC-siRNA and SPOCD1-siRNA 2. Scale bar in G: 20 μm. ^aP < 0.05; ^bP < 0.01.