SPOC domain-containing protein 1 regulates the proliferation and apoptosis of human spermatogonial stem cells through adenylate kinase 4

Figure 3 Expression pattern of SPOC domain-containing protein 1 in normal adult testis. A: Western blotting show SPOC domain-containing protein 1 (SPOCD1) levels in three obstructive azoospermia (OA) samples with normal spermatogenesis; B: Representative hematoxylin and eosin-stained image of OA testicular tissues; C: Immunohistochemistry images for cell distribution of SPOCD1 in OA samples with normal spermatogenesis; D: Double immunostaining shows co-expression of SPOCD1 with glial cell line-derived neurotrophic factor family receptor alpha-1 (GFRA1), KIT, and proliferating cell nuclear antigen (PCNA) in testes with normal spermatogenesis; E: The abundance of SPOCD1+ cells co-expressing GFRA1, KIT, and PCNA. Each circle represents one count result, and at least 20 cross-sections of seminiferous tubules were assessed for each sample. Scale bars in B and D: 50 μm.