Tumor necrosis factor-α inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation

Figure 4 Detection of human adipose-derived stem cells chondrogenic differentiation marker proteins and tumor necrosis factor-alpha and matrix metalloproteinase 3 performed after treatment with chondrogenic differentiation medium containing etanercept. A: Western blot detection of Col-2 and Aggrecan of adipose-derived stem cells treated with chondrogenic differentiation medium containing etanercept; B: Toluidine blue staining; C: Elisa detection of the tumor necrosis factor-alpha and matrix metalloproteinase 3 (MMP-3) concentration in cell supernatant; D: Western blot detection of MMP-3 and NF-κB pathway. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. MMP-3: Matrix metalloproteinase 3; NF-κB: Nuclear factor kappa-B; GM: Human adipose-derived stem cells (hADSCs) grown in growth medium alone; CH: hADSCs treated with chondrogenic differentiation medium; CHE: hADSCs treated with chondrogenic differentiation medium and 1 μg/mL etanercept.