Tumor necrosis factor-α inhibition restores matrix formation by human adipose-derived stem cells in the late stage of chondrogenic differentiation

Figure 1 Human adipose-derived stem cells flow cytometry analysis results and differentiation ability identification. A: Schematic diagram of TNF-α interferes with Human adipose-derived stem cells (hADSCs) chondrogenic differentiation; B: Flow cytometer identification of surface molecules of hADSCs: CD44, CD29, CD45, CD34, and CD105; C: Oil red O staining and Western blot detection of marker proteins performed after lipogenic differentiation induction of hADSCs; D: Alizarin red, alkaline phosphatase staining, and Western blot detection of osteogenic differentiation marker proteins performed after osteogenic differentiation of hADSCs. MMP-3: Matrix metalloproteinase 3; NF-κB: Nuclear factor kappa-B; ECM: Extracellular matrix; GM: Human adipose-derived stem cells grown in growth medium alone.