Profile of biological characterizations and clinical application of corneal stem/progenitor cells

Table 2 Methods for the isolation of limbal stem cells

Experimenter	Tissue	Isolation methods	Medium	Markers
Yamamoto et al[42], 2010	Human corneal epithelial stem/progenitor cells	0.25% collagenase and accumax	Serum-free PCT corneal epithelium medium	P75 ntr2, p63, integrin β-1
Albert et al[171], 2012	Human cornea limbal epithelial stem cells	None	Human lens capsule	KRT8, KRT14, KRT3, KRT12, KRT19, VIM, MKI67, p63α, ABCG2, CK19, Vim, Itgα9, CK8/18, CK14
Shaharuddin et al[43], 2014	Human limbal epithelial cell	LSP protocol, Hoechst 33342 dye, Flow Cytometry Analysis and Fluorescence Activated Cell Sorting	None	ABCG2, ΔNp63, Sox2
Nam et al[44], 2013	Beagle limbal epithelial cells	None	Canine amniotic membrane	ABCG2, p63, vimentin
Moriyama et al[45], 2014	Equine corneal epithelial stem cells	Dispase II and a 0.25% trypsin EDTA solution	Standard supplemented hormone epithelial medium	P63, CK14
Nam et al[46], 2015	Beagle limbal epithelial cells	Dispase II, trypsin	Canine amniotic membrane; atelocollagen gel; temperature-responsive culture dish	Ki-67, K3, ABCG2, P63
López-Paniagua et al[60], 2016	Human cornea limbal epithelial stem cells	0.25% trypsin-EDTA	Biosafe IOBA-HS culture medium	K14, K15, ABCG2, p63α
Shirzadeh et al[61], 2018	Human cornea limbal epithelial stem cells	Collagenase II and cold trypsin	Supplemental hormonal epithelial medium with husscs or 3T3 cells as feeder layers	ABCG2, ΔNP63-α, CK19, CK 3, CK12
Li et al[62], 2021	Human cornea limbal epithelial stem cells	0.05% trypsin/1 mm EDTA solution	Standard supplemented hormone epithelial medium	TSPAN7, SOX17

SHEM: Supplemented hormone epithelial medium.