Maternal inappropriate calcium intake aggravates dietary-induced obesity in male offspring by affecting the differentiation potential of mesenchymal stem cells

Figure 2 Abnormal dietary calcium intake during pregnancy and lactation affected the weight and morphology of adipose tissues among male offspring. A: Weights of the eWAT, iWAT, KAT and BAT; B: Percentage of eWAT, iWAT, KAT and BAT in body weight; C–G: Morphology of adipocytes in eWAT, iWAT, KAT and BAT by hematoxylin and eosin staining. All pooled data was represented as mean ± standard error (n = 10/group). One-way analysis of variance (ANOVA) was performed to compare the differences among the above four groups, and then Student–Newman–Keuls was involved to determine the differences between each two groups. Compared to the NC-C group, ^aP < 0.05. Compared to the NC-HFD group, ^bP < 0.05. NC-C: Normal-calcium reproductive diet and normal-fat diet after weaning; NC-HFD: Normal-calcium reproductive diet and high-fat-diet (HFD) after weaning; DC-HFD: Deficient-calcium reproductive diet and HFD after weaning; LC-HFD: Low-calcium reproductive diet and HFD after weaning; HC-HFD: High-calcium reproductive diet and HFD after weaning; eWAT: Epididymal white adipose tissue; iWAT: Inguinal white adipose tissue; KAT: Kidney adipose tissue; BAT: Brown adipose tissue.