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©The Author(s) 2021.
World J Stem Cells. Aug 26, 2021; 13(8): 1134-1150
Published online Aug 26, 2021. doi: 10.4252/wjsc.v13.i8.1134
Published online Aug 26, 2021. doi: 10.4252/wjsc.v13.i8.1134
Table 3 Effects of disease-conditioned medium on in vitro cultured stem cells
Ref. | Disease/condition | Conditioned medium/culture supernatant | Target cultured cells | Results/effects |
Raimondo et al[30], 2019 | Multiple myeloma (MM) | Exosomes from MM.1S cell line. Exosomes from bone marrow | hTERT-MSC (human telomerase reverse transcriptase stromal cell line) | Osteoclastogenesis (Osteoclast formation). Blockade of osteogenic differentiation of MSC with increased IL-8 expression |
Bougaret et al[31], 2017 | Obesity-associated increased risk of metastasis in breast cancer post-menopausal patients | Supernatant of MCF-7 cells cocultured with mature adipocytes | Human umbilical vein endothelial cells (HUVECs) | Increased HUVEC cell proliferation, endothelial tube formation, migration, IL-6 levels and decrease in Leptin and VEGFR HUVEC receptors |
Bai et al[32], 2016 | Trichinella spiralis skeletal muscle infection | Supernatant of murine macrophage cell line J774A.1 infected with ML-ESP | Murine myoblast cell line C2C12 | Avoid formation of myotubes by decreased MyoD, myogenin and MyHC protein expression affecting the differentiation of myoblasts. Suppresses the cell cycle influencing p21 and D1 protein expression |
Kozakowska et al[33], 2015 | Diabetes-associated angiogenesis deficiency | Supernatant of C2C12 myoblasts overexpressing HO-1 or a luciferase gene (control) | Intramuscular injection in mice | Improved skeletal muscle regeneration after ischemia by increased neovascularization with lower number of granulocytes and higher proportion of lymphocytes |
Wobus et al[34], 2015 | Breast cancer | Supernatant of MFC-7, MDA-MB231 breast carcinomas with respect to MCF-10 (control) | Primary Mesenchymal Stromal Cells (MSC) and MSC cell line SCP-1 | Modulate genes belonging to proliferation and differentiation. Downregulation of chemokine receptor CXCL12 expression |
Cabbage et al[35], 2014 | Atherosclerosis | Supernatant of the macrophage cell line (RAW 264.7) inoculated with Chlamydia pneumoniae | BSMC (bovine aortic smooth muscle cells) and perivascular Sca-1+, CD31-, CD45- cells from apoE-/- mouse aortas | Accelerate the conversion of smooth muscle cells to calcifying cells. Increase the production of collagen II causing osteochondrocytic phenotypes |
Fasslrinner et al[36], 2012 | Graft vs host disease/Inflammation | MLR supernatant (Mixed Lymphocyte Reaction) from PBMCs | Mesenchymal stromal cells (MSC) from the bone marrow | Decrease in the population doubling compared to control. Increased IL-8, IL-6 and Ang-1 levels. Induced osteogenic differentiation and suppress adipogenic differentiation |
Zhao et al[37], 2007 | Amyotrophic lateral sclerosis (ALS) | SOD1-G93A ALS mice CNS extracts | hMSC (human mesenchymal stem cell) | Morphological change from fibroblast to neuron phenotype without detectable apoptosis and death. Delay in proliferation of hMSC due to increased differentiation rate |
Vaisman et al[38], 1996 | Granulopoiesis in Anorexia Nervosa | Plasma and supernatant of cultured PBMCs | Bone marrow GM-CSF (granulocyte macrophage colony forming cells) | Decreased numbers of GM-CFS |
Roodman et al[39], 1992 | Paget’s disease | Supernatant of cultured Paget’s nonadherent bone marrow mononuclear cells | Bone marrow cells from healthy donors | Increased MNC (multinucleated cell) formation and IL-6 levels |
- Citation: Monzón-Nomdedeu MB, Morten KJ, Oltra E. Induced pluripotent stem cells as suitable sensors for fibromyalgia and myalgic encephalomyelitis/chronic fatigue syndrome. World J Stem Cells 2021; 13(8): 1134-1150
- URL: https://www.wjgnet.com/1948-0210/full/v13/i8/1134.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v13.i8.1134