Heat shock protein 20 promotes sirtuin 1-dependent cell proliferation in induced pluripotent stem cells

Figure 3 Heat shock protein 20 induced the expression of pluripotent genes. Real-time polymerase chain reaction showed that overexpression of heat shock protein 20 (HSP20) in the transfected induced pluripotent stem cells (iPSCs) exhibited higher expression of sex determining region Y-box 2, homeobox transcription factor nanog, octamer-binding transcription factor 4, Kruppel-like factor 4, and stage-specific embryonic antigen 4 genes compared to the iPSC control. Data are expressed as the mean ± SD and were analyzed by the Student’s t-test, ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001.