Basic Study
Copyright ©The Author(s) 2021.
World J Stem Cells. Jun 26, 2021; 13(6): 659-669
Published online Jun 26, 2021. doi: 10.4252/wjsc.v13.i6.659
Figure 1
Figure 1 Generation and molecular analysis of induced pluripotent stem cells expressing heat shock protein 20. A: Schematic diagram of the CMV-HSP20-P2A-copGFP overexpression gene construct, in which the heat shock protein 20 (HSP20) gene is under control of the cytomegalovirus promoter; B: Morphology of induced pluripotent stem cells overexpressing HSP20; C: Expression of green fluorescent protein (GFP) in passage 3; D: Expression of GFP in passage 18; E: Fluorescence enzyme-linked immunoassay image showing the expression of HSP20; F: Western blot showing the protein expression of HSP20; G: Flow cytometry analysis confirming the expression of HSP20; H: Real-time polymerase chain reaction analysis of the HSP20 gene. Data are expressed as the mean ± SD and were analyzed by the Student’s t-test, aP < 0.05, bP < 0.01, and cP < 0.001.