Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates

Figure 6 Immunohistochemical analysis of NF200 and SRY-box transcription factor 2 on spinal cord sagittal sections 12 wk after transplantation (animal NPC4). A: Large image showing the center of injury (arrow). Squares show approximate localization of enlarged panels B and C; B: Enlarged image of SRY-box transcription factor 2 (SOX2) (green) and NF200 (red) staining; C: Enlarged image of synaptophysin (green) and NF200 (red) staining; D: The results of quantification of NF200 (blue) and SOX2 (magenta); binary selection; and E: Upper images: yellow dots-number of SOX2-positive cells in the high intensity zone of NF200 fluorescence (area of growth cones); Lower images: yellow dots-number of SOX2-positive cells in the low intensity zone of NF200 fluorescence. Bar size 500–1000 µm.