Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates

Figure 1 Phenotypic characterization of directly reprogrammed neural precursor cells by flow cytometry and immunocytochemistry. A: Flow cytometry: blue peaks-negative control (isotype immunoglobulins); from left to right, top to bottom: Glial fibrillary acidic protein, nestin, βIII-tubulin SRY-box transcription factor 2 (SOX2), microtubule associated protein 2 (MAP2), human leukocyte antigen (HLA)-DR; B: Nestin and glial fibrillary acidic protein staining (most cells are double positive); C: βIII-tubulin (green); D: βIII-tubulin (green) and SOX2 (red); E: MAP2 (red) and SOX2 (green) (D and E: Partial spontaneous differentiation of directly reprogrammed neural precursor cells on laminin/poly-L-lysine coated plastic). In all panels, nuclei are counterstained with Hoechst (blue). Scale bar, 50 μm (B, C, and E) and 100 μm (D).