SmartFlareTM is a reliable method for assessing mRNA expression in single neural stem cells

Figure 3 SmartFlare^TM detection in 15 d in vitro neural stem cells. A and B: SmartFlare^TM CD133 and Oct4 probes showed a dramatic fluorescence downregulation that was limited to small cytoplasmic granules in less than half of the observed cells; C: SmartFlare^TM probe for Actin showed a robust and overall localization of red fluorescence as indicative of positive mRNA expression; D: Scramble probe-treated cells were almost completely lacking in unspecific red background. Nuclei were stained by Hoechst dye. Scale bar: 50 μm.