MKK7-mediated phosphorylation of JNKs regulates the proliferation and apoptosis of human spermatogonial stem cells

Figure 3 The influence of mitogen-activated protein kinase kinase 7 knockdown on the apoptosis of human spermatogonial stem cells. A-C: Flow cytometry and fluorescein isothiocyanate (FITC) Annexin V analysis of the percentages of early (A and B) and late (A and C) apoptosis cells in human spermatogonial stem cells (SSCs) transfected with the Control-small interfering RNA (siRNA) and mitogen-activated protein kinase kinase 7 (MKK7)siRNA 3; D and E: Terminal deoxynucleotidyl transferase nick-end-labeling (TUNEL) assays of the percentages of TUNEL⁺ cells in human spermatogonial stem cells transfected with the Control-siRNA and MKK7-siRNA 3. Scale bars in D: 50 μm. ^aP < 0.05 denotes a significant difference between the MKK7-siRNA 3 and Control-siRNA groups. dUTP: Deoxyuridine 5’-triphosphate; DAPI: 4,6-diamidino-2-phenylindole.