ARPE-19 conditioned medium promotes neural differentiation of adipose-derived mesenchymal stem cells

Figure 4 Immunofluorescence quantification for neural markers in each adipose-derived stem cells group at day 1 (white columns) and day 8 (dark columns). A: Nestin; B: NSE; C: PGP9.5; D: GFAP. ASCs: Control adipose-derived stem cells (ASCs) cultured in basal Dulbecco's Modified Eagle Medium (DMEM); sfASCs: ASCs cultured in serum-free DMEM; F12/ASCs: ASCs cultured in serum-free DMEM/F12; CM/ASCs: ASCs cultured in serum-free DMEM/F12 conditioned from ARPE-19. Bars represent CTCF mean values ± SD, obtained from at least three independent experiments. ^aSignificant differences (P < 0.01) vs ASCs; ^bSignificant differences (P < 0.01) between CM/ASCs and DMEM/F12. CTCF: Corrected total cell fluorescence; NSE: Neuron specific enolase; PGP9.5: Protein gene product 9.5; GFAP: Glial fibrillary acidic protein; sfASCs: Serum-free adipose-derived stem cells; CM: Conditioned medium.