ARPE-19 conditioned medium promotes neural differentiation of adipose-derived mesenchymal stem cells

doi:10.4252/wjsc.v13.i11.1783

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Nov 26, 2021; 13(11): 1783-1796
Published online Nov 26, 2021. doi: 10.4252/wjsc.v13.i11.1783

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Figure 2 Nestin immunoreactivity in different samples of adipose-derived stem cells cultures. Nestin (red fluorescence) detected after 1 d and 8 d of culture of human adipose-derived stem cells (ASCs). ASCs: Control ASCs cultured in basal Dulbecco's Modified Eagle Medium (DMEM); sfASCs: ASCs cultured in serum-free DMEM; F12/ASCs: ASCs cultured in serum-free DMEM/F12; CM/ASCs: ASCs cultured in serum-free DMEM/F12 conditioned from ARPE-19. Blue fluorescence indicates DAPI staining of cell nuclei. Scale bar: 50 μm. sfASCs: Serum-free adipose-derived stem cells; CM: Conditioned medium.

Citation: Mannino G, Cristaldi M, Giurdanella G, Perrotta RE, Lo Furno D, Giuffrida R, Rusciano D. ARPE-19 conditioned medium promotes neural differentiation of adipose-derived mesenchymal stem cells. World J Stem Cells 2021; 13(11): 1783-1796
URL: https://www.wjgnet.com/1948-0210/full/v13/i11/1783.htm
DOI: https://dx.doi.org/10.4252/wjsc.v13.i11.1783