Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation

Figure 9 IF-C2C12 Exos down-regulated C2C12 fibrogenic/myogenic differentiation-related proteins in vitro. A-D: MYHC, MYOD1, and MYOG protein levels in C2C12 medium were determined by western blot after incubation with different concentrations of C2C12-Exos for 24 h (1 × 10⁹, 1 × 10¹⁰, 2 × 10¹⁰/medium) (n = 4); E-G: Col 1 and α-smooth muscle actin. Data are presented as mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001. α-SMA: α-smooth muscle actin.