Exosomes derived from inflammatory myoblasts promote M1 polarization and break the balance of myoblast proliferation/differentiation

Figure 6 IF-C2C12-Exos stimulated C2C12 proliferation in vitro. A: Cell proliferation ability of C2C12 was determined using the BrdU incorporation assay after 24 h IF-C2C12-Exos incubation. Green signal = BrdU. Scale bar = 180 μm; B: The cell migration ability of C2C12 was determined by the wound-healing assay after 24 h IF-C2C12-Exos incubation; C and D: Quantification of BrdU and wound-healing data (n = 3). Data are presented as mean ± SD. ^aP < 0.05, ^bP < 0.01; E: Cell proliferation ability of C2C12 was further determined using the CCK-8 assay. Data are presented as mean ± SD. ^aP < 0.05.