Impact of senescence on the transdifferentiation process of human hepatic progenitor-like cells

Figure 7 ATP and nicotinamide adenine dinucleotide (NAD)/NAD with hydrogen content in HepaRG cells at passage 10 or passage 20 in basal conditions or after the transdifferentiation protocol (dimethyl sulfoxide). A: Cellular ATP content was expressed as ATP concentration/cells; B: Intracellular nicotinamide adenine dinucleotide (NAD⁺) and NAD with hydrogen (NADH) were extracted, and NAD⁺ and NADH levels were measured using a microplate reader. NAD⁺/NADH ratio was calculated based on the concentration of NAD⁺ and NADH. Data in the graphs are represented as the mean ± standard deviation of three independent experiments. Statistical differences were assessed by two-way analysis of variance followed by the Tukey’s test as the post hoc test. ^aP < 0.05; ^bP < 0.01. DMSO: Dimethyl sulfoxide; P10: Passage 10; P20: Passage 20.