Impact of senescence on the transdifferentiation process of human hepatic progenitor-like cells

Figure 6 Measurement of mitochondrial respiration in HepaRG cells at passage 10 or passage 20 in basal conditions or after the transdifferentiation protocol (dimethyl sulfoxide). A: Representative oxymetric traces of mitochondrial respiration in HepaRG cells. Where indicated, the following were added: 4 × 10⁶ HepaRG cells, 8 μg/mL oligomycin (OL), 2 μg/mL valinomycin (VAL), 3 mmol/L potassium cyanide (KCN). The continuous line represents the oxygen concentration measured every 0.1 s throughout the time-course of the assay; B: Representative graph of the normalized and KCN-insensitive-corrected oxygen consumption rates measured under resting respiration (RR) conditions, in the presence of OL and in the presence of VAL (see panel A); C: ATP-dependent oxygen consumption measured as absolute difference between that obtained in the absence and that in the presence of oligomycin (RR-OL); D: Respiratory control ratios obtained dividing the oxygen consumption rates measured under resting conditions by that in the presence of oligomycin (RR/OL). Data in the graphs are represented as the mean ± standard deviation of three independent experiments. Statistical differences were assessed by two-way analysis of variance followed by the Tukey’s test as the post hoc test. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. DMSO: Dimethyl sulfoxide; P10: Passage 10; P20: Passage 20.