Effect of glycyrrhizic acid and 18β-glycyrrhetinic acid on the differentiation of human umbilical cord-mesenchymal stem cells into hepatocytes

doi:10.4252/wjsc.v13.i10.1580

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250

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122

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Oct 26, 2021 (publication date) through Nov 24, 2024

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Oct 26, 2021; 13(10): 1580-1594
Published online Oct 26, 2021. doi: 10.4252/wjsc.v13.i10.1580

Table 2 qRT-PCR program layout

Process	Temperature (°C)	Time duration	Cycles
Initial denaturation	95	10 min	1
Cyclic denaturation	95	15 s	40
Annealing and extension	60	1 min	40
Melting curve	95	15 s	-
	60	15 s
	95	15 s

Citation: Fatima A, Malick TS, Khan I, Ishaque A, Salim A. Effect of glycyrrhizic acid and 18β-glycyrrhetinic acid on the differentiation of human umbilical cord-mesenchymal stem cells into hepatocytes. World J Stem Cells 2021; 13(10): 1580-1594
URL: https://www.wjgnet.com/1948-0210/full/v13/i10/1580.htm
DOI: https://dx.doi.org/10.4252/wjsc.v13.i10.1580