Copyright
©The Author(s) 2021.
World J Stem Cells. Oct 26, 2021; 13(10): 1580-1594
Published online Oct 26, 2021. doi: 10.4252/wjsc.v13.i10.1580
Published online Oct 26, 2021. doi: 10.4252/wjsc.v13.i10.1580
Process | Temperature (°C) | Time duration | Cycles |
Initial denaturation | 95 | 10 min | 1 |
Cyclic denaturation | 95 | 15 s | 40 |
Annealing and extension | 60 | 1 min | |
Melting curve | 95 | 15 s | - |
60 | 15 s | ||
95 | 15 s |
- Citation: Fatima A, Malick TS, Khan I, Ishaque A, Salim A. Effect of glycyrrhizic acid and 18β-glycyrrhetinic acid on the differentiation of human umbilical cord-mesenchymal stem cells into hepatocytes. World J Stem Cells 2021; 13(10): 1580-1594
- URL: https://www.wjgnet.com/1948-0210/full/v13/i10/1580.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v13.i10.1580