Transcriptomic alterations underline aging of osteogenic bone marrow stromal cells

Figure 2 Histological assessment of bone properties and osteogenic bone marrow stromal cells during bone aging. A: Mouse lumbar spine at different ages was sectioned and stained with hematoxylin and eosin for osteoblast detection, or underwent tartrate-resistant acid phosphatase staining for quantifying the number of osteoclasts. The quantitative analysis measured the osteoblast number per trabecular surface area or perimeter, and osteoclast number per vertebra; B: Self-renewal and differentiation capacity of bone marrow stromal cells assessed via colony-forming assay and alkaline phosphatase staining. Two peaks at 1 mo and 15 mo were detected, suggested the modeling and remodeling phase during development and aging. H&E: Hematoxylin and eosin; TRAP: Tartrate-resistant acid phosphatase; ALP: Alkaline phosphatase; N.ob: Number of osteoblasts; T.Ar: Trabecular bone area; B.pm: Bone perimeter; N.oc: Number of the osteoclast.