Involvement of glycated albumin in adipose-derived-stem cell-mediated interleukin 17 secreting T helper cell activation

Figure 5 Inhibitory effects of anti-receptor for advanced glycated end products monoclonal antibody on interleukin 17A production. Lean adipose-derived stem cells (ASC) were co-cultured with mononuclear cells (MNC) at the 1:5 ASC to MNC cell ratio, in the presence of 1% glycated human serum albumin for 48 h, phytohemagglutinin A was added. Anti-receptor for advanced glycated end products monoclonal antibody was added at a concentration of 20 µg/mL. ELISA were performed to measure interleukin (IL)-17A, interferon gamma, tumor necrosis factor alpha and IL-6 secretion. Bars represent the mean ± SE of 5 independent experiments performed in triplicates. ^aP < 0.05, as obtained by paired t tests. ASC: Adipose-derived stem cells; MNC: Mononuclear cells; G-HSA: Glycated human serum albumin; PHA: Phytohemagglutinin A; RAGE: Receptor for advanced glycated end products; mAb: Monoclonal antibody; IL: Interleukin; IFNγ: Interferon gamma; TNFα: Tumor necrosis factor alpha.