Involvement of glycated albumin in adipose-derived-stem cell-mediated interleukin 17 secreting T helper cell activation

Figure 3 Glycated human serum albumin increases receptor for advanced glycated end products and human leukocyte antigen-DR expression in adipose-derived stem cell / mononuclear cell co-cultures. Lean adipose-derived stem cells (ASC) were co-cultured with mononuclear cells (MNC) at the 1:5 ASC to MNC cell ratio, in the presence of 1% glycated human serum albumin or human serum albumin (HSA) for 48 h, phytohemagglutinin A (PHA) was added or not. As control MNC were cultured alone in the presence or not of PHA, and HSA. Human leukocyte antigen-DR, receptor for advanced glycated end products, cluster of differentiation (CD) 41, CD62P, and intercellular adhesion molecule 1 were stained with fluorescent-conjugated antibodies, and analyzed by cytofluorometry, using the DIVA software. This experiment is representative of two experiments performed, with two different ASC. ASC: Adipose-derived stem cells; MNC: Mononuclear cells; G-HSA Glycated human serum albumin; HSA: Human serum albumin; PHA: Phytohemagglutinin A; HLA: Human leukocyte antigen; RAGE: Receptor for advanced glycated end products; CD: Cluster of differentiation; ICAM-1: Intercellular adhesion molecule 1; FITC: Fluorescein isothiocyanate; PE: Phycoerythrin; APC: Allophycocyanin.