CR6-interacting factor-1 contributes to osteoclastogenesis by inducing receptor activator of nuclear factor κB ligand after radiation

Figure 6 CR6-interacting factor-1-protein kinase cyclic adenosine monophosphate-activited catalytic subunit alpha interaction model and the inhibition potential of the lowest toxic effect of compounds. A: CR6-interacting factor-1 (Crif1)-protein kinase cyclic adenosine monophosphate-activited catalytic subunit alpha (PRKACA) interaction model showing Crif1 (colored in rose red) and PRKACA (colored in cyan). Interface amino acids are shown as sticks and colored in rose red (for Crif1) and cyan (for PRKACA) and indicated as a zoomed-in view in the inset figure; B-F: Chemical structure of each inhibitor molecule and their docked pose on Crif1 (colored in rose red, surface view). Docked molecule (stick) and the amino acids involved in the hydrophobic interactions (light purple) are shown. F0382-0033 (B), F3408-0076 (C), F1430-0134 (D), F3408-0031 (E), and F1430-0130 (F); G-K: A tetrazolium salt (WST-8) assay was carried out to study the toxic effect of compounds on the H-BM-MSCs. F0382-0033 (G), F3408-0076 (H), F1430-0134 (I), F3408-0031 (J), and F1430-0130 (K). The bars represent the mean ± standard deviation. H-BM-MSCs: Human bone marrow mesenchymal stem/stromal cells.