C-C chemokine receptor type 2-overexpressing exosomes alleviated experimental post-stroke cognitive impairment by enhancing microglia/macrophage M2 polarization

Figure 4 Exo^CCR2 drove microglia/macrophage M2 polarization and inhibited microglia/macrophage M1 polarization at day 4 and day 14 after transient middle cerebral occlusion compared to Exo^Ctrl. A-D: Relative CD16, IL-1β, CD206, and Arg-1 mRNA expression changes in samples obtained from rats in each group on day 4 after transient middle cerebral occlusion (tMCAO), n = 6, ^aP < 0.05, ^bP < 0.01, ^cP < 0.001; E-H: Relative CD16, IL-1β, CD206, and Arg-1 mRNA expression changes in samples obtained from rats in each group on day 14 after tMCAO, n = 6, ^bP < 0.01, ^cP < 0.001; I: CD16/iba-1 immunofluorescence staining and cell colocalization counts 14 d after tMCAO. Scale bar = 50 μm, n = 6, ^aP < 0.05, ^cP < 0.001; J: CT206/iba-1 immunofluorescence staining and cell colocalization counts 14 d after tMCAO. Scale bar = 50 μm, n = 6, ^aP < 0.05, ^cP < 0.001.