C-C chemokine receptor type 2-overexpressing exosomes alleviated experimental post-stroke cognitive impairment by enhancing microglia/macrophage M2 polarization

Figure 1 Human umbilical cord mesenchymal stem cells^CCR2 load the C-C chemokine receptor type 2 receptor into their exosomes. A, B: Flow cytometry analysis of the C-C chemokine receptor type 2 (CCR2) receptor on human umbilical cord mesenchymal stem cells (HUC-MSCs)^Ctrl and HUC-MSCs^CCR2, n = 3, ^cP < 0.001; C: CCR2 mRNA expression in HUC-MSCs^Ctrlvs HUC-MSCs^CCR2, n = 3, ^cP < 0.001; D: Western blotting analysis for the quantification of the CCR2 expression in HUC-MSCs^Ctrlvs HUC-MSCs^CCR2, n = 3; E, F: Analysis of the exosomal morphology and diameter distribution of Exo^Ctrl and Exo^CCR2 using transmission electron microscopy and the qNano® system, respectively, n = 3; G: Western blotting analysis for the detection of the exosomal specific markers CD9, CD63, and CD81 in Exo^Ctrl and Exo^CCR2, n = 3; H: Western blotting analysis for the quantification of the exosomal CCR2 expression in the Exo^Ctrl and Exo^CCR2 samples, n = 3; I: Schematic diagram describing the extraction of the exosomes from the medium; J: Detection of the CCL2-binding ability of the exosomes by ELISA, n = 3, ^cP < 0.001.