Clonal isolation of endothelial colony-forming cells from early gestation chorionic villi of human placenta for fetal tissue regeneration

Figure 5 Chorionic villus endothelial colony-forming cells can be transduced by lentiviral vector and are compatible with a small intestinal submucosa scaffold. A: Representative images of chorionic villus endothelial colony-forming cells (CV-ECFCs) and cord blood-derived ECFCs (CB-ECFCs) transduced with a pCCLc-MNDU3-LUC-PGK-Tomato-WPRE lentiviral vector. Flow cytometric analysis showed a transduction rate of 85.1% and 82.8%, respectively; B: tdTomato lentiviral vector-transduced CV-ECFCs (left panel) and CB-ECFCs (right panel) seeded onto a small intestinal submucosa extracellular matrix scaffold, showing adherence. Scale bar = 100 µm.