Effects of normobaric cyclic hypoxia exposure on mesenchymal stem-cell differentiation–pilot study on bone parameters in elderly

Figure 4 Increased hypoxia time in cyclic hypoxia inhibits lipid-droplet formation. Mesenchymal stem cells induced to differentiate into adipocytes were exposed to cyclic hypoxia during 1, 2 or 4 h/d (3% O₂), four days per week. Cultures were stained with oil-red O to reveal lipid droplets at day 13. A: representative images of oil-red O staining of cultures differentiating into adipocytes, in normoxia or cyclic hypoxia with 1, 2 or 4 h of hypoxia; and B: Oil-red O quantification. ^aP < 0.05 vs normoxia (0 h of hypoxia).