Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells

Figure 3 Adipogenesis differentiation analysis. A: Representative images of noninduced (control) and adipogenic-induced human adipose-derived stromal/stem cells (hASCs) from young hASC (yASC) and old hASC (oASC) donors for 7 d, 14 d and 21 d. Lipid droplets were stained green with Nile red fluorescent dye, and nuclei were stained blue by DAPI (Scale bar: 100 μm). B: Quantitative analysis was performed by measuring the fluorescence of Nile red stain (fluorescence microplate reader); C: Quantitative analysis was performed by measuring the intracellular stained area with Nile red (Operetta^®); D: Expression levels of CEBPA and PPARγ2 mRNA after adipogenic induction of yASCs and oASCs. Data are represented as the mean ± SD and are compared using a t test. NS: Nonsignificant differences.