Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells

Figure 1 Immunophenotypic characterization of young and old human adipose-derived stromal/stem cells. A: Representative flow cytometry histograms of surface markers in human adipose-derived stromal/stem cells (hASCs) derived from young and old donors. Blue lines represent the young hASCs (yASCs) results, in which blue tinted histograms represent the stained cells and single lines represent specific isotype controls. Orange-dotted lines represent the old hASCs (oASCs) results, in which orange tinted histograms represent the stained cells and single dotted lines represent specific isotype controls; B: Quantification of the percentage of positive cells for each surface marker (yASCs: n = 9, oASCs: n = 9). Each bar represents the mean ± SD, ^aP < 0.05; C: Representative images of yASC and oASC morphology after 3 d and 7 d of culture. Scale bar = 200 µm.