Stem cells from human exfoliated deciduous teeth ameliorate concanavalin A-induced autoimmune hepatitis by protecting hepatocytes from apoptosis

Figure 3 Pretreatment with stem cells from human exfoliated deciduous teeth pretreatment inhibits concanavalin A-induced hepatocyte apoptosis. A: Terminal dUTP nick-end labeling staining of apoptotic cells; B: Semi-quantitative analysis of the percentage of terminal dUTP nick-end labeling⁺ area in liver tissues. Arrows indicate positive cell death in the liver section; C: NCTC-1469 liver cells in the indicated treatment groups. NCTC cells are labeled with PKH26; D: Quantitative analysis of NCTC-1469 liver cell number; E: NCTC cell apoptosis detected by flow cytometry; F: Quantitative analysis of Annexin V⁺PI^- rate; G: Quantitative analysis of Annexin V⁺PI⁺ rate. Scale bars = 100 μm. ^aP < 0.05, ^bP < 0.01, n ≥ 3.