6-gingerol protects nucleus pulposus-derived mesenchymal stem cells from oxidative injury by activating autophagy

Figure 3 6-Gingerol alleviates hydrogen peroxide-induced decrease of nucleus pulposus-derived mesenchymal stem cell viability and intracellular reactive oxygen species level. A: The viability of nucleus pulposus-derived mesenchymal stem cells (NPMSCs) treated with different concentrations of 6-gingerol (6-GIN) was detected by cholecystokinin-8 (CCK-8); B: CCK-8 results of NPMSCs treated with different concentrations of hydrogen peroxide; C: Effect of 6-GIN on the NPMSC viability induced by hydrogen peroxide; D: Statistical analysis of the production of intracellular reactive oxygen species [NPMSCs treated with cell culture medium (CON group), 80 μmol/L hydrogen peroxide alone (HYD group), hydrogen peroxide and 30 μmol/L 6-GIN (6-GIN group), or hydrogen peroxide and 6-GIN combined with 10 mmol/L 3-methyladenine (3-MA group)]. All data are the mean ± SE of at least three independent experiments. ^aP < 0.05 vs 0 μmol/L 6-GIN group; ^bP < 0.05 vs 0 μmol/L H₂O₂ group; ^cP < 0.05 vs 0 μmol/L blank control; ^dP < 0.05 vs 80 μmol/L H₂O₂ group; ^eP < 0.05 vs CON group; ^fP < 0.05 vs HYD group; ^gP < 0.05 vs 6-GIN group. 6-GIN: 6-gingerol; ROS: Reactive oxygen species; H₂O₂: Hydrogen peroxide; 3-MA: 3-Methyladenine.