Acupuncture accelerates neural regeneration and synaptophysin production after neural stem cells transplantation in mice

Figure 3 Neural stem cell proliferation by immunofluorescence staining. Paraffin section of hippocampus tissue of mice was evaluated by indirect immunofluorescence and diaminobenzidine staining. After nuclei were counterstained with hematoxylin, positive BrdU-labeled cells were observed and counted. Arrows indicate positive cells marked by BrdU expression. A: Immunofluorescence staining; B: BrdU positive cell counting. ^aP < 0.05 when compared to senescence-accelerated mouse prone 8 (SAMP8) neural stem cells (NSCs) transplantation group, ^bP < 0.05 when compared to SAMP8 NSCs transplantation with non-acupoint group. RC: Senescence-accelerated mouse resistant 1 control group; PC: Senescence-accelerated mouse prone 8 control group; PS: Senescence-accelerated mouse prone 8 sham operation group; PT: Senescence-accelerated mouse prone 8 neural stem cells transplantation group; PTA: Senescence-accelerated mouse prone 8 neural stem cells transplantation with acupuncture group; PTN: Senescence-accelerated mouse prone 8 neural stem cells transplantation with non-acupoint group.