Basic Study
Copyright ©The Author(s) 2020.
World J Stem Cells. Dec 26, 2020; 12(12): 1667-1690
Published online Dec 26, 2020. doi: 10.4252/wjsc.v12.i12.1667
Table 1 Primer sequences and product sizes
Gene
Primer sequence (5’ —> 3’)
Product size (bp)
Runt-related transcription factor 2 (RUNX2)TGGTTAATCTCCGCAGGTCAC; ACTGTGCTGAAGAGGCTGTTTG143
Osterix (SP7)AGCCAGAAGCTGTGAAACCTC; AGCTGCAAGCTCTCCATAACC163
Collagen, type I, alpha 1 (COL1A1)CGCTGGCCCCAAAGGATCTCCTG; GGGGTCCGGGAACACCTCGCTC263
Integrin-binding sialoprotein (IBSP)AGGGCAGTAGTGACTCATCCG; CGTCCTCTCCATAGCCCAGTGTTG171
Osteocalcin (BGLAP)CCATGAGAGCCCTCACACTCC; GGTCAGCCAACTCGTCACAGTC258
Vascular endothelial growth factor A (VEGFA)GCGAGCAGCGAAAGCGACAGG; CGTCAGCGCGACTGGTC114
Peroxisome proliferator-activated receptor gamma 2 (PPARG2)GCGATTCCTTCACTGATACACTG; GAGTGGGAGTGGTCTTCCATTAC136
Fatty acid synthase (FASN)AAGCTGAAGGACCTGTCTAGG; CGGAGTGAATCTGGGTTGATG146
Adipose triglyceride lipase (ATGL) or patatin-like phospholipase domain-containing protein 2 (PNPLA2) CCAACACCAGCATCCAGTTCA; ATCCCTGCTTGCACATCTCTC102
Glycerol-3-Phosphate Dehydrogenase 1 (GPD1)ATACAGCATCCTCCAGCACAAG; GGATGATTCTGCAGGCAGTG120
Peroxisome proliferator-activated receptor gamma, coactivator 1 alpha (PPARGC1A)CTGGTTGCCTGCATGAGTGTG; GGGCAAAGAGGCTGGTCTTCAC173
Osteoprotegerin (OPG)GGCGCTACCTTGAGATAGAGTTCTG; TGTTTTCTACAGGGTGCTTTAGATGAC160
Receptor activator for nuclear factor kappa B ligand (RANKL)CGTCGCCCTGTTCTTCTATTTC; AAATGCAGTGAGTGCCATCTTC74
Low-density lipoprotein receptor related protein 5 (LRP5)TACTGGACAGACTGGCAGACC; GTGTAGAAAGGCTCGCTTGG209
Low-density lipoprotein receptor related protein 6 (LRP6)TACTGGCCAAATGGACTGACT; TGTTGCAAGCCAAAATGGAGT211
Dickkopf Wnt signaling pathway inhibitor 1 (DKK1)ATGCGTCACGCTATGTGCT; GGAATACCCATCCAAGGTGCTA144
Polymerase RNA II polypeptide A (POLR2A)TTTTGGTGACGACTTGAACTGC; CCATCTTGTCCACCACCTCTTC125