Effect of conditioned medium from neural stem cells on glioma progression and its protein expression profile analysis

Figure 4 Recombinant human NCAN protein increases glioma cell proliferation and invasion. A: The proliferation of glioma cells was significantly increased after treatment with different concentrations of recombinant human NCAN (rhNCAN), as measured by CCK8 assay, especially at the concentrations of 20 and 50 ng/mL; B and C: rhNCAN (both 20 and 50 ng/mL) significantly improved the invasion ability of U87 and U251 glioma cells; D: Expression of NCAN in the human neural stem cells and glioma cells detected by Western blot. β-actin was used as the loading control. -1 and -2 indicated two independent samples; E: After treatment of U87 and U251 glioma cells with rhNCAN (20 and 50 ng/mL), the downstream RhoA-ROCK signaling pathway was significantly activated in both glioma cells, as measured by Western blot. β-actin was used as the loading control. Con: Control 10% FBS condition medium group; rhNCAN: Recombinant human NCAN protein. (^aP < 0.05 vs Control, ^bP < 0.01 vs Control).