Effect of conditioned medium from neural stem cells on glioma progression and its protein expression profile analysis

Figure 1 Characteristics of human neural stem cells. A: The proliferation morphology of human neural stem cells (hNSCs) cultured in vitro from Day 1 to Day 6. The hNSCs presented a neurosphere morphology; B: Immunofluorescence for hNSCs specific markers, including Nestin, SOX2, and Msil1. DAPI indicated the cell nucleus; C: The differentiation markers of hNSCs, including Tuj1 (a neuron marker), GFAP (an astrocyte marker), and MOG (an oligodendrocyte marker); D: Western blot analysis showing that after culturing in hypoxic (3% O₂) condition, oxygen-glucose deprived-hNSCs overexpressed the hypoxic marker HIF-1α. hNSCs: Human neural stem cells; Tuj1: β-tubulin III; GFAP: Glial fibrillary acidic protein; MOG: Myelin oligodendrocyte glycoprotein.