AlCl3 exposure regulates neuronal development by modulating DNA modification

Figure 3 AlCl₃ exposure alters the levels of DNA 5-hydroxymethylcytosine, 5-methylcytosine, and N⁶-methyladenine in adult neural stem cells and neurons. A-C: Representative images of 5-hydroxymethylcytosine (5hmC), 5-methylcytosine (5mC), and N⁶-methyladenine (6mA) dot-blot assays in adult neural stem cell (aNSC) differentiation and proliferation; D-F: Quantification revealing that the relative levels of 5-mc and 6mA both decreased in aNSC proliferation and differentiation, but the level of 5hmc increased (n = 3); G-I: Representative images of 5hmC, 5mC, and 6mA dot-blot assays in neurons; J-L: Quantification revealing that the relative levels of 5hmC decreased but those of 5mC and 6mA increased in neurons (n = 3). Data are represented as the mean ± SE (n = 3). Statistically significant differences are indicated: ^aP < 0.05, ^bP < 0.01, ^cP< 0.001. 5hmC: 5-hydroxymethylcytosine; 5mC: 5-methylcytosine; 6mA: N⁶-methyladenine.