Copyright
©The Author(s) 2020.
World J Stem Cells. Oct 26, 2020; 12(10): 1152-1170
Published online Oct 26, 2020. doi: 10.4252/wjsc.v12.i10.1152
Published online Oct 26, 2020. doi: 10.4252/wjsc.v12.i10.1152
Figure 8 Evaluation of the barrier integrity by trans-endothelial electrical resistance in human retinal endothelial cells monocultures or in co-cultures of human retinal endothelial cells with pericytes (human retinal pericyte cells) or different groups of human adipose-derived mesenchymal stem cells.
A: Trans-endothelial electrical resistance values measured on day 3; B: Trans-endothelial electrical resistance values measured on day 6. Trans-endothelial electrical resistance values measured on day 3 (A) and day 6 (B) show that significant increases were observed only when human retinal endothelial cells (hRECs) were co-cultured with human adipose-derived mesenchymal stem cells pre-cultured in complete pericyte medium or human retinal pericyte cells. Values are expressed as Ohm/cm2 and calculated by the formula: (Average resistance of experimental wells - average resistance of blank wells) × 0.33 (area of the transwell membrane). Values were gathered from three independent experiments carried out in duplicate (n = 6). The boxes define the 25th and 75th percentile; the center line is the median; the whiskers are the range. aIndicates significant difference (P < 0.05) vs hRECs; bIndicates significant difference (P < 0.05) vs hASCs pre-stimulated with transforming growth factor; cIndicates significant difference (P < 0.05) vs hASCs pre-cultured in pericyte medium lacking pericyte growth supplement. hASCs: Human adipose-derived mesenchymal stem cells pre-cultured in basal medium; hRECs: Human retinal endothelial cells; hRPCs: Human retinal pericyte cells; TGF-hASCs: hASCs pre-stimulated with transforming growth factor; noPGS-hASCs: hASCs pre-cultured in pericyte medium lacking pericyte growth supplement; PM-hASCs: hASCs pre-cultured in complete pericyte medium.
- Citation: Mannino G, Gennuso F, Giurdanella G, Conti F, Drago F, Salomone S, Lo Furno D, Bucolo C, Giuffrida R. Pericyte-like differentiation of human adipose-derived mesenchymal stem cells: An in vitro study. World J Stem Cells 2020; 12(10): 1152-1170
- URL: https://www.wjgnet.com/1948-0210/full/v12/i10/1152.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v12.i10.1152