Enrichment of retinal ganglion and Müller glia progenitors from retinal organoids derived from human induced pluripotent stem cells - possibilities and current limitations

doi:10.4252/wjsc.v12.i10.1171

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Oct 26, 2020; 12(10): 1171-1183
Published online Oct 26, 2020. doi: 10.4252/wjsc.v12.i10.1171

Table 1 qPCR primers

Primer	Forward sequence 5’-3’	Reverse sequence 5’-3’	Accession number
GAPDH_1	AGGGCTGCTTTTAACTCTGGT	CCCCACTTGATTTTGGAGGGA	NM_001289745.2
GAPDH_2	GAAGGTGAAGGTCGGAGTCAA	GGAACATGTAAACCATGTAGTTGAG	NM_002046
Ki67	GGGCGAAGTTCACAGTCAAT	CTCCTTCACTGGGGTCTTGA	NM_001145966.1
Nestin	CAGGAGAAACAGGGCCTACA	AAGCTGAGGGAAGTCTTGGA	NM_006617.1
RAX	GAACAGCCCAAGAAAAAGCA	GCTTCATGGAGGACACTTCC	NG_013031.1
VSX2	AAGGATGGCATCATGGACTC	TCAGTTCCTCCTGGGAGATG	NG_013092.1
GFAP	AAGAGATCCGCACGCAGTAT	AGGTCAAGGACTGCAACTGG	NM_001242376.1
RLBP1	CAACTGGAGCAGCTCACAAC	TCCTTCTCTTGCACCCTCTC	XM_011521870.2
CD44	CCCAGATGGAGAAAGCTCTG	CCTGAAGTGCTGCTCCTTTC	NM_001001391
CD117	GCAAATACACGTGCACCAAC	GCACCCCTTGAGGGAATAAT	NM.001093772
BRN3A	5'CGGGGAGCCATAATCTGCAA3’	TTTCGACTCAGTTCGTGCGT3	NM_006237.4

Citation: Freude KK, Saruhanian S, McCauley A, Paterson C, Odette M, Oostenink A, Hyttel P, Gillies M, Haukedal H, Kolko M. Enrichment of retinal ganglion and Müller glia progenitors from retinal organoids derived from human induced pluripotent stem cells - possibilities and current limitations. World J Stem Cells 2020; 12(10): 1171-1183
URL: https://www.wjgnet.com/1948-0210/full/v12/i10/1171.htm
DOI: https://dx.doi.org/10.4252/wjsc.v12.i10.1171