Regeneration of the central nervous system-principles from brain regeneration in adult zebrafish

Figure 4 Glial cell reactivity and tissue restoration in the adult zebrafish telencephalon in response to stab wound injury. A: Representative scheme of cell composition in the intact telencephalon of adult zebrafish; B-D: Cellular response to mechanical injury in the adult zebrafish telencephalon; B: Injury-induced cell death stimulates the activation of inflammatory response. ATP is sensed by microglial cells (blue), through P2Y12 receptor, triggering their change in morphology and their migration, together with oligodendroglial precursor cells (OPCs, brown) at the injury site (blue circle); C: Microglial and oligodendroglial cell accumulation is resolved and intermediate progenitors (light blue), source of newly formed neurons (yellow), populate the injury site, where they start differentiating into neurons. Proliferation reaches its peak in the ventricular zone, where stem cells (ependymoglial cells, light green) reside. This cellular response seems to be required to increase the neuronal output and to re-populate the loss of progenitors in the subventricular zone, due to their migration to compensate the neuronal loss at the injury site; D: Regeneration is efficiently completed, stem cell activation returns to basal levels, surviving newborn neurons are found at the injury site and manage to survive, no signs of glial scar can be found; E: Scheme of genes specifically regulated in ependymoglial cells, relevant for their activation state and the promotion of restorative neurogenesis.