Generation of induced secretome from adipose-derived stem cells specialized for disease-specific treatment: An experimental mouse model

Figure 3 Animal study design and comparison of systemic effects in each group. A: Animal study design. Control mice and TAA-treated mice were intravenously (using tail vein) infused with normal saline, conditioned media (CM), and induced CM (iCM); B: Serology tests of aspartate transaminase, alanine transaminase, and creatinine in TAA-treated mice. iCM infusion decreased the serum levels of aspartate transaminase, alanine transaminase, and creatinine to the greatest extent; C: Results of ELISA showing serum levels of inflammatory markers (IL-6 and TNF-α) in each group. iCM administration lowered the serum levels of IL-6 and TNF-α the most in TAA-treated mice. Values are presented as mean ± SD of three independent experiments. ^cP < 0.05 vs control (saline); ^eP < 0.05 between CM and iCM. ALT: Alanine transaminase; AST: Aspartate transaminase; iCM: Isecretome group of which components were the TAA-induced secretome; NCM: Normal conditioned media group of which components were the naïve secretome; PECAM: Platelet endothelial cell adhesion molecule; TAA: Thioacetamide.