Sphere-forming corneal cells repopulate dystrophic keratoconic stroma: Implications for potential therapy

Figure 2 Cellular repopulation of the surface of en face keratoconic tissue sections. A: A stem cell-enriched sphere (arrow) was seeded onto a keratoconic decellularised central corneal stromal section (phase contrast image); B: Assessed for viability with Calcein-AM (green) at day 14 (fluorescence image). Montage imaging of the entire tissue section shows cells have repopulated the tissue section to complete confluence by day 14. Scale bar = 500 µm.