Sphere-forming corneal cells repopulate dystrophic keratoconic stroma: Implications for potential therapy

Table 2 Statistical analysis of difference in gene expression in sphere-seeded tissue sections as measured by droplet digital polymerase chain reaction, effect of time: day 0 vs day 14

	Day 0	Day 14	Day 14 - Day 0
Target	Estimate1 (lower.95, upper.95)	Estimate1 (lower.95, upper.95)	P
ABCB5	0.02 (0.01, 0.08)	0 (0, 0.01)	0.003	S
ABCG2	0.71 (0.25, 1.99)	0.02 (0.01, 0.05)	0.000	S
aSMA	0.21 (0.07, 0.58)	0.23 (0.09, 0.62)	0.871	NS
Col1A1	4.66 (1.65, 13.12)	4.97 (1.84, 13.44)	0.929	NS
Col1A2	11.33 (4.02, 31.92)	3.89 (1.44, 10.52)	0.145	NS
dNp63	0.03 (0.01, 0.09)	0 (0, 0)	0.000	S
ITGa3	0.12 (0.04, 0.33)	0.28 (0.10, 0.75)	0.244	NS
ITGb1	5.81 (2.06, 16.36)	7.99 (2.96, 21.59)	0.663	NS
Keratocan	0.14 (0.05, 0.39)	0.01 (0, 0.03)	0.000	S
KRT3	0.28 (0.08, 0.96)	0 (0, 0.01)	0.000	S
LAMA1	0.33 (0.12, 0.94)	0.07 (0.03, 0.19)	0.032	S
Notch1	0.43 (0.15, 1.21)	0.10 (0.04, 0.26)	0.043	S
P63a	0.05 (0.02, 0.15)	0 (0, 0.01)	0.000	S
PCNA	0.52 (0.19, 1.48)	0.71 (0.26, 1.92)	0.681	NS
Vimentin	16.96 (6.02, 47.77)	42.18 (15.61, 113.96)	0.214	NS

¹Values are the back-transformed estimates from the multivariate regression; NS: Not significant; S: Significant (P ≤ 0.05); ABCB5: ATP binding cassette subfamily B member 5; ABCG2: ATP-binding cassette, sub-family G member 2; αSMA: Smooth muscle, actin; COL1A1: Collagen type I, alpha 1 chain; COL1A2: Collagen type I, alpha 2 chain; TP63: Tumour protein p63; ∆NP63: Transcript variant 1, ∆NP63: N-terminal isoform, ITGa3: Integrin, subunit alpha 3; ITGB1: Integrin, subunit beta 1; KRT3: Keratin 3; LAMA1: Laminin, subunit alpha 1; TP63: Tumour protein p63; PCNA: Proliferating cell nuclear antigen.