Sphere-forming corneal cells repopulate dystrophic keratoconic stroma: Implications for potential therapy

doi:10.4252/wjsc.v12.i1.35

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Jan 26, 2020 (publication date) through Dec 24, 2024

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Jan 26, 2020; 12(1): 35-54
Published online Jan 26, 2020. doi: 10.4252/wjsc.v12.i1.35

Table 1 PrimeTime quantitative polymerase chain reaction assays used for droplet digital polymerase chain reaction

Product of interest: Gene	Reference gene or gene of interest	Assay identification	Size of complimentary DNA amplicon, base pairs
Actin, beta	Reference	Hs.PT.39a.22214847	109
Beta-2-microglobulin	Reference	Hs.PT.58v.18759587	142
Glucuronidase beta	Reference	Hs.PT.58v.27737538	127
Glycreraldehyde-3-phosphate dehydrogenase	Reference	Hs.PT.39a.22214836	142
Hypoxanthine phosphoribosyltransferase 1	Reference	Hs.PT.58v.45621572	148
Peptidylprolyl isomerase A	Reference	Hs.PT.58v.38887593. g	101
Actin, alpha 2, smooth muscle, aorta	Gene of interest	Hs.PT.56a.2542642	131
ATP binding cassette subfamily B member 5	Gene of interest	Hs.PT.58.38502346	106
ATP-binding cassette, sub-family G member 2	Gene of interest	Hs.PT.58.20889058	114
Collagen type I, alpha 1 chain	Gene of interest	Hs.PT.58.15517795	130
Collagen type I, alpha 2 chain	Gene of interest	Hs.PT.58.26714160	108
Integrin, subunit beta 1	Gene of interest	Hs.PT.58.39883300	141
Keratin 3	Gene of interest	Hs.PT.58.25330614	126
Keratocan	Gene of interest	Hs.PT.58.45594658	130
Laminin, subunit alpha 1	Gene of interest	Hs.PT.58.3170022	129
Notch1	Gene of interest	Hs.PT.58.23074795	112
Proliferating cell nuclear antigen	Gene of interest	Hs.PT.58.4761611	94
Tumour protein p63, transcript variant 1, C-terminal isoform α	Gene of interest	Hs.PT.58.39019253	108
Tumour protein p63, transcript variant 1, N-terminal isoform	Gene of interest	Hs.PT.58.19710922	104
Vimentin	Gene of interest	Hs.PT.58.38906895	141

Citation: Wadhwa H, Ismail S, McGhee JJ, Van der Werf B, Sherwin T. Sphere-forming corneal cells repopulate dystrophic keratoconic stroma: Implications for potential therapy. World J Stem Cells 2020; 12(1): 35-54
URL: https://www.wjgnet.com/1948-0210/full/v12/i1/35.htm
DOI: https://dx.doi.org/10.4252/wjsc.v12.i1.35