Triple-modal imaging of stem-cells labeled with multimodal nanoparticles, applied in a stroke model

Figure 4 Internalization and viability analysis of human bone marrow mesenchymal stem cells_Luc labeled with multimodal nanoparticles-IR750. A-N: Optical microscopy images of hBM-MSC_Luc labeled with MNP-IR750, shown at 10 × (left column images) and 40 × (right column images); O: Cellular viability of hBM-MSC_Luc after labeling in MTT (red bars) and BLI (blue bars) assays. Internalization of MPN-IR750 was performed at the following concentrations: 5, 10, 20, 30, 40 and 50 µg Fe/mL. hBM-MSC: Human bone marrow mesenchymal stem cells; MNP: Multimodal nanoparticles; MTT: The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide; BLI: Bioluminescent images.