Developments in cell culture systems for human pluripotent stem cells

Table 1 Primed human pluripotent stem cell culture conditions

	Thomson et al[1]	Xu et al[53]	Xu et al[20]	Vallier et al[16]	Li et al[26]	Ludwig et al[30,80]	Liu et al[52]	Lu et al[33]	Yao et al[117]	Wang et al[23]	Chen et al[17]	Frank et al[118]
FGF2	-	+	+	+	+	+	+	+	+	+	+	+
TGF-β family	-	-	-	Activin	-	TGF-β	-	TGF-β	-	Activin	TGF-β/Nodal	TGF-β/Activin
Serum	FBS	KOSR	KOSR	XF	-	-	-	-	-	-	-	-
Other factors	-	-	Noggin	Insulin	FLT3	Insulin	-	Insulin, BAFF, WNT3A	-	LR3-IGF1, HRG1β	Insulin	Dorsomorphin, IWP-2
Common culture supplements	-	-	NEAA, BME, L-glutamine	BSA, L-glutamine, transferrin, BME, F12, Monothio-glycerol	BME, NEAA, L-glutamine	BSA, BME, transferrin, cholesterol, pipecolic acid, GABA	BME, N2, B27, L-glutamine	BME, transferrin, cholesterol, albumin, L-glutamine	BME, N2, B27, NEAA, BSA-Fraction-V, L-glutamine	BSA-Fraction-V, NEAA, Vc, transferrin, BME, trace elements A, B, C	Vc, transferrin, selenium, NaHCO3	N2, B27, BSA, L-glutamine, NEAA, BME
Base medium	DMEM/F12	DMEM	DMEM/F12	IMDM	XVIVO-10	DMEM/F12	DMEM/F12	DMEM/F12	DMEM/F12	DMEM/F12	DMEM/F12	DMEM/F12
ECM	-	Matrigel	Matrigel	Matrigel	Matrigel	Matrigel	Fibronectin	Matrigel	Matrigel	Matrigel	Matrigel/ Vitronectin	Matrigel
Feeder	MEF	-	-	-	-	-	-	-	-	-	-	-
Markers	Surface markers[119]: SSEA3, SSEA4, TRA1-60, TRA1-81, CD24, CD57, CD90
	Nuclear markers: OCT4, NANOG, SOX2