Unmodified autologous stem cells at point of care for chronic myocardial infarction

Figure 5 Microvessel density after delivery of UA-ADRCs or saline. The panels show representative photomicrographs of paraffin-embedded, 5 µm thick tissue sections of post mortem hearts from pigs in group 1 (delivery of fresh, uncultured, unmodified, autologous adipose-derived regenerative cells) (A, B) and group 2 (delivery of saline as control) (C, D) at T2. In (A, C), tissue sections were stained with Masson’s Trichrome staining. In (B, D), tissue sections were processed with fluorescence immunohistochemistry in order to detect von Willebrand factor (red) (counterstaining with DAPI in blue). The arrows point to microvessels, and the arrowheads to small arterioles. The scale bar shown in D represents 100 µm in (A, C) and 35 µm in (B, D). UA-ADRCs: Fresh, uncultured, unmodified, autologous adipose-derived regenerative cells.