Regenerative potential of mouse embryonic stem cell-derived PDGFRα+ cardiac lineage committed cells in infarcted myocardium

Figure 2 Implantations of platelet-derived growth factor receptor-α⁺ cardiac lineage-committed cells and αMHC⁺ cardiomyocytes equally improves contractile function and structure in the infarcted heart. A: Representative M-mode transthoracic echocardiography views of control, myocardial infarction (MI), MI+ platelet-derived growth factor receptor-α (PDGFRα)⁺ cardiac lineage-committed cells (CLCs), and MI+αMHC⁺ cardiomyocytes (CMs). Improved anterior (white arrowheads) and septal (yellow arrowheads) regional wall motion are observed in the left ventricles of MI+PDGFRα⁺ CLCs and MI+αMHC⁺ CMs; B and C: Quantifications of left ventricular internal dimension in systole (mm), ejection fraction (%) and fractional shortening (%). Each group, n = 7. ^aP < 0.05 and ^bP < 0.01 vs Con; ^cP < 0.01 vs MI; D: Gross images of hearts in control, MI, MI+PDGFRα⁺ CLCs, and MI+αMHC⁺ CMs. Scale bars, 2.5 mm; E: H and E staining of mid-sectioned hearts of control, MI, MI+PDGFRα⁺ CLCs, and MI+αMHC⁺ CMs. Scale bars, 1 mm and 50 μm in the upper and lower panels, respectively; F: Quantifications of the thickness (mm) of left ventricle in the infarcted region. Each group, n = 7. ^bP < 0.01 vs Con; ^cP < 0.01 vs MI or MI+PDGFRα⁺ CLCs. CLCs: Cardiac lineage-committed cells; CMs: Cardiomyocytes; MI: Myocardial infarction.