Regenerative potential of mouse embryonic stem cell-derived PDGFRα+ cardiac lineage committed cells in infarcted myocardium

Figure 1 Implantations of platelet-derived growth factor receptor-α⁺ cardiac lineage-committed cells and αMHC⁺ cardiomyocytes in the acute myocardial infarction murine model. A: Experimental scheme of implanting either platelet-derived growth factor receptor-α (PDGFRα)⁺ cardiac lineage-committed cells (CLCs) or αMHC⁺ cardiomyocytes (CMs) into acute myocardial infarction (MI) murine model. Analyses were performed at 2 wk after implantation of approximately 1 × 10⁶ cells of PDGFRα⁺ CLCs or αMHC⁺ CMs into the left ventricular myocardium of acute MI murine model; B: Live cell image showing tdTomato⁺ cells during induction of PDGFRα⁺ CLCs from embryonic stem cells. Scale bars, 100 μm; C: Representative confocal image showing implanted tdTomato⁺ PDGFRα⁺ CLCs in the myocardial spaces (arrowheads) of the infarcted zone (dotted line and arrow), which was formed by ligation of coronary artery 1 h prior to the implantation. Scale bar, 500 μm. CLCs: Cardiac lineage-committed cells; CMs: Cardiomyocytes; PSCs: Pluripotent stem cells; ESCs: Embryonic stem cells; LIF: Leukemia inhibitory factor.