NF-κB promotes the stem-like properties of leukemia cells by activation of LIN28B

Figure 4 Role of LIN28B in nuclear factor kappa B (NF-B)-mediated leukemic stem cell-like properties. A: RNAs of pEGFP and pEGFP-LIN28B transfected TF-1a cells, followed by qRT-PCR comparing LIN28B transcription level. The expression of LIN28B in each sample was normalized with GAPDH respectively (n = 3, mean ± SD); B: A total of 2000 cells from TF-1a or LIN28B overexpression cells were initially plated in methylcellulose medium containing either DMSO as control or MG-132 at 50 nmol/L, followed by another two rounds of serial replating assays. Quantification of colonies of indicated cells over 3 rounds of replating in methylcellulose medium. Bar figures represented 3 separated experiments (n = 3, mean ± SD). ^aP < 0.05; ^bP < 0.01.